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Index > Protein center > Scap(Gene name) > Mouse
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  • Scap (Gene name),
  • Sterol regulatory element-binding protein cleavage-activating protein (Protein name ),  SCAP_MOUSE from NCBI database.
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  • General Annotation
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  • Gene name:
    Scap(Kiaa0199);
    Protein name:
    Sterol regulatory element-binding protein cleavage-activating protein(SCAP;SREBP cleavage-activating protein);
    Alternative:

    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Membrane region forms a homotetramer. Forms a stable complex with SREBF1/SREBP1 or SREBF2/SREBP2 through its C-terminal cytoplasmic domain. Forms a ternary complex with INSIG1 or INSIG2 through its transmembrane domains at high sterol concentrations. Interacts with the SEC23/SEC24 complex in a SAR1-GTP-dependent manner through an ER export signal in its third cytoplasmic loop. Binds cholesterol through its SSC domain. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts with RNF139; the interaction inhibits the interaction of SCAP with SEC24B and hampering the ER to Golgi transport of the SCAP/SREBP complex.
    Function:
    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
    Subcellular Location:
    Endoplasmic reticulum membrane Multi-pass membrane protein Golgi apparatus membrane Multi-pass membrane protein Cytoplasmic vesicle COPII-coated vesicle membrane Multi-pass membrane protein Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    Protein Attributes:
    Sequence length:
    1276
    Sequence:
    50:
    MTLTERLREK | ISQAFYNHGL | LCASYPIPII | LFTGLCILAC | CYPLLKLPLP | 
    100:
    GTGPVEFSTP | VKGYSPPPAD | SDHKQGEPSE | QPEWYVGAPV | AYIQQIFVKS | 
    150:
    SVSPWHRNLL | AVDVFRSPLS | RAFQLVEEIR | NHVLRDSSGT | KSLEEVCLQV | 
    200:
    TDLLPGLRKL | RSLLPEHGCL | LLSPGNFWQN | DWERFHADPD | IIGTIHQHEP | 
    250:
    KTLQTSATLK | DLLFGVPGKY | SGVSLYTRKR | MVSYTITLVF | QRYHAKFLSS | 
    300:
    LRARLMLLHP | SPNCSLRAEN | LVHVHFKEEI | GIAELIPLVT | TYIILFAYIY | 
    350:
    FSTRKIDMVK | SKWGLALAAV | VTVLSSLLMS | VGLCTLFGLT | PTLNGGEIFP | 
    400:
    YLVVVIGLEN | VLVLTKSVVS | TPVDLEVKLR | IAQGLSSESW | SIMKNAATEL | 
    450:
    GIILIGYFTL | VPAIQEFCLF | AVVGLVSDFF | LQMLFFTTVL | SIDIRRMELA | 
    500:
    DLNKRLPPES | CLPSAKPVGR | PARYERQQAV | RPSTPHTITL | QPSSFRNLRL | 
    550:
    PKRLRVIYFL | ARTRLAQRLI | MAGTVVWIGI | LVYTDPAGLR | TYLAAQVTEQ | 
    600:
    SPLGEGSLGP | MPVPSGVLPA | SHPDPAFSIF | PPDAPKLPEN | QTLPGELPEH | 
    650:
    AGPAEGVHDS | RAPEVTWGPE | DEELWRKLSF | RHWPTLFNYY | NITLAKRYIS | 
    700:
    LLPVIPVTLH | LNPREALEGR | HPQDGRSAWA | PQEPLPAGLW | ESGPKGPGGT | 
    750:
    QTHGDITLYK | VAALGLAAGI | VLVLLLLCLY | RVLCPRNYGQ | PGGGPGRRRR | 
    800:
    GELPCDDYGY | APPETEIVPL | VLRGHLMDIE | CLASDGMLLV | SCCLAGQVCV | 
    850:
    WDAQTGDCLT | RIPRPGPRRD | SCGGGAFETQ | ENWERLSDGG | KASPEEPGDS | 
    900:
    PPLRRRPRGP | PPPSLFGDQP | DLTCLIDTNF | SVQLPPEPTQ | PEPRHRVGCG | 
    950:
    RSRDSGYDFS | RLVQRVYQEE | GLAAMRMPAL | RPPSPGPPLP | QASQEEGTAP | 
    1000:
    EKGSPPLAWT | PSTAGSIWSL | ELQGNLIVVG | RSSGRLEVWD | AIEGVLCCSN | 
    1050:
    EEISSGITAL | VFLDRRIVAA | RLNGSLDFFS | LETHTSLSPL | QFRGTPGRGS | 
    1100:
    SPSSSVYSSS | NTVTCHRTHT | VPCAHQKPIT | ALRAAAGRLV | TGSQDHTLRV | 
    1150:
    FRLDDSCCLF | TLKGHSGAIT | AVYIDQTMVL | ASGGQDGAIC | LWDVLTGSRV | 
    1200:
    SQTFAHRGDV | TSLTCTASCV | ISSGLDDFIS | IWDRSTGIKL | YSIQQDLGCG | 
    1250:
    ASLGVISDNL | LVTGGQGCVS | FWDLNYGDLL | QTVYLGKNSE | AQPARQILVL | 
    1276:
    DNAAIVCNFG | SELSLVYVPS | VLEKLD
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    Pfam:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse SCAP
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    CLIA Kit for Mouse SCAP
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    CLIA Kit for Mouse SCAP
    Cat.:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse SCAP
    Cat.:
    U15518m
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse SCAP
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    CLIA Kit for Mouse SCAP
    Cat.:
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    Packing:
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    Polyclonal Antibody for Mouse SCAP
    Polyclonal Antibody for Mouse SCAP
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    P15518Rb-h
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    Packing:
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    Packing:
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    Polyclonal Antibody for Mouse SCAP
    Monoclonal Antibody for Mouse SCAP
    Monoclonal Antibody for Mouse SCAP
    Monoclonal Antibody for Mouse SCAP
    Monoclonal Antibody for Mouse SCAP
    Monoclonal Antibody for Mouse SCAP
    Protein for Mouse SCAP
    Protein for Mouse SCAP
    Protein for Mouse SCAP
    Protein for Mouse SCAP
    Protein for Mouse SCAP

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    References
    1. 1.
      "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N. , Kikuno R. , Ohara R. , Inamoto S. , Koseki H. , Hiraoka S. , Saga Y. , Seino S. , Nishimura M. , Kaisho T. , Hoshino K. , Kitamura H. , Nagase T. , Ohara O. , Koga H.
      DNA Res.11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Fetal brain.
    2. 2.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Embryonic heart.
      tissue: Macrophage.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6.
      strain: FVB/N.
      tissue: Brain.
      tissue: Colon.
      tissue: Mammary gland.
    4. 4.
      "Blunted feedback suppression of SREBP processing by dietary cholesterol in transgenic mice expressing sterol-resistant SCAP(D443N)."
      Korn B.S. , Shimomura I. , Bashmakov Y. , Hammer R.E. , Horton J.D. , Goldstein J.L. , Brown M.S.
      J. Clin. Invest.102:2050-2060(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF ASP-443
    5. 5.
      "SREBP cleavage-activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation."
      Matsuda M. , Korn B.S. , Hammer R.E. , Moon Y.-A. , Komuro R. , Horton J.D. , Goldstein J.L. , Brown M.S. , Shimomura I.
      Genes Dev.15:1206-1216(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-821 AND SER-934;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
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