Gene name:
Scap (Kiaa0199) ;
Protein name:
Sterol regulatory element-binding protein cleavage-activating protein (SCAP;SREBP cleavage-activating protein) ;
Alternative:
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Membrane region forms a homotetramer. Forms a stable complex with SREBF1/SREBP1 or SREBF2/SREBP2 through its C-terminal cytoplasmic domain. Forms a ternary complex with INSIG1 or INSIG2 through its transmembrane domains at high sterol concentrations. Interacts with the SEC23/SEC24 complex in a SAR1-GTP-dependent manner through an ER export signal in its third cytoplasmic loop. Binds cholesterol through its SSC domain. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts with RNF139; the interaction inhibits the interaction of SCAP with SEC24B and hampering the ER to Golgi transport of the SCAP/SREBP complex.
Function:
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
Subcellular Location:
Endoplasmic reticulum membrane
Multi-pass membrane protein
Golgi apparatus membrane
Multi-pass membrane protein
Cytoplasmic vesicle
COPII-coated vesicle membrane
Multi-pass membrane protein
Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Protein Attributes:
50:
MTLTERLREK | ISQAFYNHGL | LCASYPIPII | LFTGLCILAC | CYPLLKLPLP |
100:
GTGPVEFSTP | VKGYSPPPAD | SDHKQGEPSE | QPEWYVGAPV | AYIQQIFVKS |
150:
SVSPWHRNLL | AVDVFRSPLS | RAFQLVEEIR | NHVLRDSSGT | KSLEEVCLQV |
200:
TDLLPGLRKL | RSLLPEHGCL | LLSPGNFWQN | DWERFHADPD | IIGTIHQHEP |
250:
KTLQTSATLK | DLLFGVPGKY | SGVSLYTRKR | MVSYTITLVF | QRYHAKFLSS |
300:
LRARLMLLHP | SPNCSLRAEN | LVHVHFKEEI | GIAELIPLVT | TYIILFAYIY |
350:
FSTRKIDMVK | SKWGLALAAV | VTVLSSLLMS | VGLCTLFGLT | PTLNGGEIFP |
400:
YLVVVIGLEN | VLVLTKSVVS | TPVDLEVKLR | IAQGLSSESW | SIMKNAATEL |
450:
GIILIGYFTL | VPAIQEFCLF | AVVGLVSDFF | LQMLFFTTVL | SIDIRRMELA |
500:
DLNKRLPPES | CLPSAKPVGR | PARYERQQAV | RPSTPHTITL | QPSSFRNLRL |
550:
PKRLRVIYFL | ARTRLAQRLI | MAGTVVWIGI | LVYTDPAGLR | TYLAAQVTEQ |
600:
SPLGEGSLGP | MPVPSGVLPA | SHPDPAFSIF | PPDAPKLPEN | QTLPGELPEH |
650:
AGPAEGVHDS | RAPEVTWGPE | DEELWRKLSF | RHWPTLFNYY | NITLAKRYIS |
700:
LLPVIPVTLH | LNPREALEGR | HPQDGRSAWA | PQEPLPAGLW | ESGPKGPGGT |
750:
QTHGDITLYK | VAALGLAAGI | VLVLLLLCLY | RVLCPRNYGQ | PGGGPGRRRR |
800:
GELPCDDYGY | APPETEIVPL | VLRGHLMDIE | CLASDGMLLV | SCCLAGQVCV |
850:
WDAQTGDCLT | RIPRPGPRRD | SCGGGAFETQ | ENWERLSDGG | KASPEEPGDS |
900:
PPLRRRPRGP | PPPSLFGDQP | DLTCLIDTNF | SVQLPPEPTQ | PEPRHRVGCG |
950:
RSRDSGYDFS | RLVQRVYQEE | GLAAMRMPAL | RPPSPGPPLP | QASQEEGTAP |
1000:
EKGSPPLAWT | PSTAGSIWSL | ELQGNLIVVG | RSSGRLEVWD | AIEGVLCCSN |
1050:
EEISSGITAL | VFLDRRIVAA | RLNGSLDFFS | LETHTSLSPL | QFRGTPGRGS |
1100:
SPSSSVYSSS | NTVTCHRTHT | VPCAHQKPIT | ALRAAAGRLV | TGSQDHTLRV |
1150:
FRLDDSCCLF | TLKGHSGAIT | AVYIDQTMVL | ASGGQDGAIC | LWDVLTGSRV |
1200:
SQTFAHRGDV | TSLTCTASCV | ISSGLDDFIS | IWDRSTGIKL | YSIQQDLGCG |
1250:
ASLGVISDNL | LVTGGQGCVS | FWDLNYGDLL | QTVYLGKNSE | AQPARQILVL |
1276:
DNAAIVCNFG | SELSLVYVPS | VLEKLD
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Pig
Mouse
Rat
Human
Bovine
Polyclonal Antibody for Mouse SCAP
Polyclonal Antibody for Mouse SCAP
Polyclonal Antibody for Mouse SCAP
Polyclonal Antibody for Mouse SCAP
Polyclonal Antibody for Mouse SCAP
Monoclonal Antibody for Mouse SCAP
Monoclonal Antibody for Mouse SCAP
Monoclonal Antibody for Mouse SCAP
Monoclonal Antibody for Mouse SCAP
Monoclonal Antibody for Mouse SCAP
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
1.
"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
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DNA Res.11:205-218(2004)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue :
Fetal brain .
2.
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more...
Science309:1559-1563(2005)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain :
C57BL/6J .
tissue :
Embryonic heart .
tissue :
Macrophage .
3.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain :
C57BL/6 .
strain :
FVB/N .
tissue :
Brain .
tissue :
Colon .
tissue :
Mammary gland .
4.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;MUTAGENESIS OF ASP-443
5.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;DISRUPTION PHENOTYPE
6.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-821 AND SER-934;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Liver .